Photochemically-Enabled, Post-Translational Production of Therapeutic C-Terminal Amides”
Time: 2:30 pm
day: Conference Day 2
Details:
- We have developed chemistry to convert recombinantly expressed peptides containing a C-terminal cysteine extension to a therapeutically relevant C-terminal amide via photochemical decomposition of the corresponding arylated cysteine thiol
- The process is robust, high-yielding, amenable to UV- or visible-light conditions, and is broadly applicable to all natural amino acid side chains at the C-terminus. We also demonstrate the applicability to a range of biologically relevant peptides
- Finally, we show that the photochemical process is amenable to flow-based methods at gram and decagram scale, indicating potential applicability to CMC processes alongside current methodologies dependent on resource intensive expression methods such as PAM enzyme
